Tsis ntev los no, Associate Professor Li Jiawen pawg nyob rau hauv Micro thiab Nano Engineering Laboratory, Tsev Kawm Ntawv ntawm Engineering Science, University of Science thiab Technology ntawm Tuam Tshoj (USTC) tau npaj siab femtosecond laser dynamic holographic ua txoj kev rau kev tsim kho ntawm 3D capillary scaffolds, uas tuaj yeem ua tau. siv los tsim 3D capillary networks. Cov hauj lwm tau luam tawm raws li "Kev tsim kho sai ntawm 3D Biomimetic Capillary Networks nrog Complex Morphology Siv Dynamic Holographic Processing Cov haujlwm tau luam tawm hauv Cov Khoom Siv Ua Haujlwm Zoo Tshaj Plaws hauv qab lub npe ntawm "Kev Tsim Kho sai ntawm 3D Biomimetic Capillary Networks nrog Complex Morphology Siv Dynamic Holographic Processing" thiab yog xaiv raws li daim ntawv npog ntawm phau ntawv journal, thiab lwm yam thev naus laus zis tau tso cai los ntawm patent.
Femtosecond laser ob-photon polymerization muaj nanoscale ua daws teeb meem thiab peb-dimensional fabrication muaj peev xwm, tab sis cov tsoos ua tswv yim los luam microvascular tes hauj lwm yog inefficient. Raws li kev ua haujlwm dhau los, cov pab pawg tau tshaj tawm txoj kev hloov kho hauv zos raws li lub nplhaib-zoo li Bessel beam los tsim lub nplhaib-shaped notched lub teeb teb, thiab siv qhov hloov ceev-hloov notched nplhaib-zoo li lub teeb kom nthuav tawm sab hauv photoresist, realizing. lub high-efficiency machining ntawm complex duab bifurcated microtubule network thiab bionic ntxeem tau microtubules, thiab lub machining ceev yog tshaj 30 lub sij hawm siab tshaj ntawm cov tsoos point-by-point machining txoj kev. Cov pab pawg siv cov ntxeem tau microtubule network ua ib qho scaffold los coj cov hlwb endothelial kom loj hlob tuaj rau ntawm phab ntsa, paub txog kev tsim kho ntawm cov kab mob microvascular nyuaj nrog cov lus txhais tau, thiab cov haujlwm no yuav muab lub platform rau kev tshawb fawb ua haujlwm hauv cov ntaub so ntswg engineering, tshuaj xyuas tshuaj. thiab vascular physiology. Bowen Song, tus kws qhia ntawv, Shengying Fan, tus kws kho mob kawm, thiab Chaowei Wang, tus kws kho mob tom qab, yog tus kws sau ntawv thawj zaug, thiab Jiawen Li yog tus sau sib xws.
Daim duab Efficient kev tsim kho ntawm microvascular network: (a) Schematic ntawm dynamic holographic npaum ua; (b) Bifurcated microtubules; (c) Endothelial hlwb nyob rau saum npoo ntawm microtubules
Nyob rau hauv xyoo tas los no, Jiawen Li pawg tau nquag tshawb nrhiav daim ntawv thov ntawm femtosecond laser processing technology hauv biomedical teb, thiab tau ua tiav hauv txoj kev npaj ntawm micro-nano robots. Micro-nano robots qhia tau hais tias muaj txiaj ntsig zoo rau kev thov hauv thaj chaw biomedicine. Txhawm rau kom paub txog qhov kev npaj loj loj thiab tswj tau kev thauj mus los ntawm micro-robots nyob rau hauv ib puag ncig nyuaj, pab pawg tau tshaj tawm txoj kev npaj ua haujlwm zoo ntawm ib puag ncig teb micro-helical robots raws li kev hloov pauv dynamic holographic teeb teb, uas tuaj yeem ua ntau txhiab hydrogel micro. -helical robots hauv 0.5h. Tus neeg hlau paub txog kev hloov pauv hloov pauv ntawm nws tus kheej morphology raws li kev tswj hwm pH, uas ua rau muaj ntau hom kev txav txav uas tau tsav los ntawm cov hlau nplaum, thiab ua tiav cov phiaj xwm thauj tshuaj (ACS Nano 2021, 15, 18048; Lub Teeb: Adv. Manufacturing 2023, 4: 29). Yuav kom daws tau qhov teeb meem ntawm cov ntsiab lus tsis tshua muaj hlau nplaum thiab lub zog tsav me me ntawm micro-helical neeg hlau, uas nyuaj rau kev kov yeej cov txiaj ntsig ntawm ib puag ncig ntws nrawm, pab pawg tau npaj cov txheej txheem raws li ob-photon polymerization tsim thiab sintering txoj kev los npaj cov ntshiab. nickel helical micro-robots, uas muaj cov ntsiab lus sib nqus ntawm li 90 wt%, txhim kho magnetic torque nyob rau hauv qis-zog rotating magnetic teb, nrog lub siab tshaj plaws ceev ntawm 12.5 lub cev ntev ib ob thiab muaj peev xwm mus propel ib yam khoom 200 lub sij hawm hnyav dua. Nws tus kheej, thiab tswj kev txav hauv cov kua dej (Lab Chip, 2024, DOI: 10.1039/d3lc01084h).
Daim duab Micro-nano kauv robots: (a) kev npaj tau zoo thiab ib puag ncig cov khoom ntawm hydrogel micro-nano neeg hlau; (b) micro-nano hlau neeg hlau tuaj yeem kov yeej cov txiaj ntsig ntawm kev khiav nrawm.
Tsis tas li ntawd, Jiawen Li pab pawg tau tshawb fawb txog cov txiaj ntsig ntawm micro-nanostructures ntawm kev loj hlob ntawm neuronal tus cwj pwm raws li femtosecond laser two-photon processing technology. Nyob rau hauv kev koom tes nrog Prof. Guo-Qiang Bi ntawm lub Department of Life Sciences thiab tshuaj thiab Associate Prof. Weiping Ding ntawm lub tsev kawm ntawv ntawm cov ntaub ntawv Science thiab Technology, lawv siv femtosecond ob-photon technology los npaj arrays ntawm patterned micropillars nrog txawv spacings thiab heights , thiab pom tias neuronal axons nyiam loj hlob ntawm isometric micropillars, thiab cov neurons tuaj yeem raug coj mus rau kev loj hlob thiab cov kab mob neural los ntawm kev tsim cov kab micropillar (Adv. Healthcare Mater. 2021, 10, 2100094). Kev tshoov siab los ntawm axonal myelination, cov pab pawg sib koom tsim thiab npaj cov qauv microtubule nrog cov kab sib txawv, phab ntsa tuab thiab ntev kom ua raws li axonal myelination, thiab pom tias cov qauv microtubule muaj peev xwm ua kom qhov kev loj hlob ntawm neuronal axons (ntau dua 10 zaug). Tsis tas li ntawd, cov pab pawg sib koom ua ke magnetically sputtered ib tug sib nqus nyias zaj duab xis ntawm npib tsib xee thiab biocompatible nyias zaj duab xis ntawm titanium nyob rau saum npoo ntawm cov microtubules, uas yuav siv tau rau qhov tseeb kev twb kev txuas ntawm neurons nyob rau hauv lub manipulation ntawm ib tug sab nraud magnetic teb los tsim ib tug tshwj xeeb lom. neural circuits (Nano Lett., 2022, 22: 8991). Cov micro-nanostructures muaj peev xwm paub txog kev loj hlob ntawm kev coj noj coj ua thiab kev loj hlob sai ntawm cov neurons, uas yuav muab cov txheej txheem thiab cov tswv yim rau kev taw qhia kev sib txuas ntawm cov paj hlwb cais, kev tsim kho ntawm neural tes hauj lwm, thiab kho cov hlab ntsha sai.
Fig. Cov txiaj ntsig ntawm micro-nano qauv ntawm kev loj hlob ntawm neuronal axon: (a) neuronal axons loj hlob raws tib qhov siab micro-pillars hauv kev taw qhia; (b) ntxeem tau microtubules ceev neuronal axon kev loj hlob thiab tuaj yeem paub txog kev sib txuas ntawm cov neurons.
Cov haujlwm tshawb fawb saum toj no tau txais kev txhawb nqa los ntawm National Natural Science Foundation ntawm Tuam Tshoj, Kev Tshawb Fawb Tseem Ceeb thiab Kev Txhim Kho ntawm Ministry of Science thiab Technology, thiab Anhui Provincial Science thiab Technology Major Research Project.
